Journal of Contemporary Agriculture and Bioscience
Use of Endophytic Bacteria in Combination of Trichoderma for Healthy Management of Root Rot Disease in Pea
Received: 02 January, 2026 || Accepted: 27 January, 2026 || Published:28 January, 2026
A b s t r a c t
Root rot disease complex, caused by multiple soil-borne pathogens, is the most destructive disease affecting pea (Pisum sativum L.) cultivation worldwide, often resulting in complete crop failure. This study evaluated the efficacy of fungal antagonist Trichoderma spp. and bacterial biofertilizer Rhizobium spp., both individually and in combination, for sustainable management of pea root rot. Three Trichoderma strains (T. viride, T. harzianum-BD, and T. harzianum-TH) were assessed for their in vitro antagonistic activity against Fusarium solani, the primary causal agent of root rot. Rhizobium spp. was isolated from lentil root nodules and cultured on Yeast Extract Mannitol Agar (YEMA) medium. Dual culture assays demonstrated significant mycelial growth inhibition of F. solani by all three Trichoderma strains, with inhibition percentages ranging from 65.4% to 78.9%. A field experiment was conducted using a Randomized Complete Block Design (RCBD) with seven treatments: T₀ (Control), T₁ (T. viride), T₂ (T. harzianum-BD), T₃ (T. harzianum-TH), T₄ (Rhizobium), T₅ (Trichoderma + Rhizobium), and T₆ (Chemical fungicide). The combined application of Trichoderma and Rhizobium (T₅) showed superior performance in reducing disease incidence and enhancing plant growth parameters compared to individual treatments. This integrated biocontrol approach offers a promising eco-friendly alternative to chemical fungicides for sustainable pea production.
Keywords : Pea root rot, Fusarium solani, Trichoderma spp., Rhizobium, Biocontrol, Integrated disease management and Seed treatment.
Copyright information: Copyright © 2026 Author(s) retain the copyright of this article. This work is licensed under a Creative Commons Attribution 4.0 International License
How to cite: Rahman, T.M.R., Salahin, M. and Akter, F. 2026. Use of Endophytic Bacteria in Combination of Trichoderma for Healthy Management of Root Rot Disease in Pea. Journal of Contemporary Agriculture and Bioscience, 3(1), 1-8.
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